How genes are physically encoded on the genome may dictate dynamics of gene expression. Given a regulatory network, many arrangements are possible, and each might exhibit different behavior depending on where/how they are located on the genome. One such example is the rhamnose regulon in E. coli. The regulatory gene RhaR and the sugar transporter RhaT are encoded arranged in such a manner that the C-terminals of the two genes overlap. In this work, we explore the effect of the resulting transcriptional interference in dictating dynamics of gene expression and sugar utilization.